EC: 1.14.11.11

hyoscyamine (6S)-dioxygenase (L-hyoscyamine,2-oxoglutarate:oxygen oxidoreductase ((6S)-hydroxylating))

enzyme lambda metabolic reaction experiment
uniprot:Q6V0J1 [hyoscyamine (6S)-dioxygenase(Enzyme) wildtype his-tagged]

2_Oxoglutarate + (6S)_Hydroxyhyoscyamine + O2 -> Succinate + H2O + CO2 + Scopolamine
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 0.4 mM FeSO4, 4 mM sodium ascorbate, 2 mg/ml catalase
PH: 7.4
Temperature: 30
uniprot:Q6V0J1 [hyoscyamine (6S)-dioxygenase(Enzyme) wildtype his-tagged]

L_Hyoscyamine + O2 + 2_Oxoglutarate -> Succinate + (6S)_Hydroxyhyoscyamine + CO2
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 0.4 mM FeSO4, 4 mM sodium ascorbate, 2 mg/ml catalase
PH: 7.4
Temperature: 30
uniprot:P24397 [Fe2+]

L_Hyoscyamine + O2 + 2_Oxoglutarate -> (6S)_Hydroxyhyoscyamine + Succinate + CO2
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5% (v/v) Acetone
PH: 7.5
Temperature: 25
uniprot:P24397 [Fe2+]

2_Oxoglutarate + O2 + L_Hyoscyamine -> Succinate + CO2 + (6S)_Hydroxyhyoscyamine
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5% (v/v) Acetone
PH: 7.5
Temperature: 25
uniprot:P24397 [Fe2+]

O2 + L_Hyoscyamine + 2_Oxoglutarate -> CO2 + (6S)_Hydroxyhyoscyamine + Succinate
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 5% (v/v) Acetone
PH: 7.5
Temperature: 25

Pathways

pathway id name