EC: 3.1.3.37

sedoheptulose-bisphosphatase (sedoheptulose-1,7-bisphosphate 1-phosphohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:P46285 [Mg2+]

Sedoheptulose_1,7_bisphosphate + H2O -> Phosphate + Sedoheptulose_7_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tricine-NaOH, 30 mM DTT
PH: 8.2
Temperature: 20
uniprot:P46285 [sedoheptulose-bisphosphatase(Enzyme) wildtype]

Sedoheptulose_1,7_bisphosphate + H2O -> Phosphate + Sedoheptulose_7_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tricine/NaOH, 10 mM MgCl2, 20 mM KCl, 0.1 mM ATP, 1 mM phosphoenolpyruvate, 0.15 mM NADH, 20 mM dithiothreitol, 2 U/ml pyruvate kinase, 2 U/ml lactate dehydrogenase, 0.5 U/ml fructose-6-phosphate kinase
PH: 8.2
Temperature: 36
uniprot:O20252 [Mg2+]

H2O + Sedoheptulose_1,7_bisphosphate -> Sedoheptulose_7_phosphate + Phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tricine-NaOH, 20 mM KCl, 0.1 mM ATP, 1 mM Phosphoenolpyruvate, 0.15 mM NADH, 30 mM Dithiothreitol, 2 U/ml Pyruvate kinase, 2 U/ml Lactate dehydrogenase, 0.5 U/ml Fructose 6-phosphate kinase
PH: 8.2
Temperature: 30
uniprot:O20252 [Mg2+]

Sedoheptulose_1,7_bisphosphate + H2O -> Phosphate + Sedoheptulose_7_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tricine-NaOH, 20 mM KCl, 0.1 mM ATP, 1 mM Phosphoenolpyruvate, 0.15 mM NADH, 30 mM Dithiothreitol, 2 U/ml Pyruvate kinase, 2 U/ml Lactate dehydrogenase, 0.5 U/ml Fructose 6-phosphate kinase
PH: 8.2
Temperature: 30
uniprot:O20252 [Mg2+]

Fructose_1,6_bisphosphate + H2O -> Fructose_6_phosphate + Phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 0.5 mM EDTA, 0.3 mM NADP, 0.6 U/ml Glucose 6-phosphate dehydrogenase, 1.2 U/ml Phosphoglucose isomerase
PH: 8.8
Temperature: 30
uniprot:O20252 [Mg2+]

Fructose_1,6_bisphosphate + H2O -> Phosphate + Fructose_6_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 0.5 mM EDTA, 0.3 mM NADP, 0.6 U/ml Glucose 6-phosphate dehydrogenase, 1.2 U/ml Phosphoglucose isomerase
PH: 8.8
Temperature: 30
uniprot:Q31QY2 [Mg2+]

Sedoheptulose_1,7_bisphosphate + H2O -> Phosphate + Sedoheptulose_7_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 0.15 mM NADH, 0.1 mM ATP, 1 mM phosphoenolpyruvate, 20 mM dithiothreitol, 0.4 mM EDTA, 20 mM KCl, 2 U/ml pyruvate kinase, 2 U/ml lactate dehydrogenase, 0.5 U/ml fructose-6-phosphate kinase
PH: 8
Temperature: 36

Pathways

pathway id name
BioCyc:META_PHOTOALL-PWY oxygenic photosynthesis
BioCyc:ARA_CALVIN-PWY Calvin-Benson-Bassham cycle
BioCyc:SYNEL_PWY-5484 glycolysis II (from fructose 6-phosphate)
BioCyc:SYNEL_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:META_CALVIN-PWY Calvin-Benson-Bassham cycle
BioCyc:SYNEL_GLYCOLYSIS-E-D superpathway of glycolysis and Entner-Doudoroff
BioCyc:META_PWY-7218 photosynthetic 3-hydroxybutanoate biosynthesis (engineered)
BioCyc:SYNEL_PHOTOALL-PWY oxygenic photosynthesis
BioCyc:META_PWY-7124 ethylene biosynthesis V (engineered)
BioCyc:ARA_PHOTOALL-PWY oxygenic photosynthesis
BioCyc:SYNEL_SUCSYN-PWY sucrose biosynthesis I (from photosynthesis)
BioCyc:SYNEL_GLUCONEO-PWY gluconeogenesis I
BioCyc:SYNEL_CALVIN-PWY Calvin-Benson-Bassham cycle
PlantCyc:PLANT_CALVIN-PWY Calvin-Benson-Bassham cycle
PlantCyc:ARA_CALVIN-PWY Calvin-Benson-Bassham cycle
PlantCyc:ARA_PHOTOALL-PWY oxygenic photosynthesis
PathBank:SMP0002406 Riboneogenesis
PathBank:SMP0012094 Calvin-Benson Cycle
PathBank:SMP0012089 Photosynthesis