EC: 2.6.1.2

alanine transaminase (L-alanine:2-oxoglutarate aminotransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q93ZN9 [alanine transaminase(Enzyme) wildtype His6-AGD2]

L_Glutamate + Pyruvate -> L_Alanine + 2_Oxoglutarate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 100 mM MgCl2, 1 µM pyridoxal-5-phosphate
PH: 8
Temperature: 37
uniprot:Q93ZN9 [alanine transaminase(Enzyme) mutant His6-AGD2 P398S]

Pyruvate + L_Glutamate -> L_Alanine + 2_Oxoglutarate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 100 mM MgCl2, 1 µM pyridoxal-5-phosphate
PH: 8
Temperature: 37
uniprot:Q9ZQI7 [alanine transaminase(Enzyme) wildtype His6-ALD1]

Pyruvate + L_Glutamate -> 2_Oxoglutarate + L_Alanine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 100 mM MgCl2, 1 µM pyridoxal-5-phosphate
PH: 8
Temperature: 37
uniprot:P34106 [Pyridoxal phosphate]

L_Glutamate + Pyruvate -> 2_Oxoglutarate + L_Alanine		 (( (Vmax * S) ) / (S + Km)) buffer: 0.2 mM NADH, 2 mM EDTA, 50 mM Hepes-KOH, 100 mM NH4Cl, 6 U glutamate dehydrogenase
PH: 7.5
Temperature: 25
uniprot:P34106 [Pyridoxal phosphate]

L_Glutamate + Pyruvate -> 2_Oxoglutarate + L_Alanine		 (( (Vmax * S) ) / (S + Km)) buffer: 0.2 mM NADH, 2 mM EDTA, 50 mM Hepes-KOH, 100 mM NH4Cl, 6 U glutamate dehydrogenase
PH: 7.5
Temperature: 25
uniprot:Q93ZN9 [alanine transaminase(Enzyme) wildtype His6-AGD2]

L_Alanine + 2_Oxoglutarate -> Pyruvate + L_Glutamate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 100 mM MgCl2, 1 µM pyridoxal-5-phosphate
PH: 8
Temperature: 37
uniprot:Q9ZQI7 [alanine transaminase(Enzyme) wildtype His6-ALD1]

L_Alanine + 2_Oxoglutarate -> Pyruvate + L_Glutamate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 100 mM MgCl2, 1 µM pyridoxal-5-phosphate
PH: 8
Temperature: 37
uniprot:Q93ZN9 [alanine transaminase(Enzyme) mutant His6-AGD2 P398S]

L_Alanine + 2_Oxoglutarate -> Pyruvate + L_Glutamate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris/HCl, 100 mM MgCl2, 1 µM pyridoxal-5-phosphate
PH: 8
Temperature: 37
uniprot:P34106 [Pyridoxal phosphate]

2_Oxoglutarate + L_Alanine -> Pyruvate + L_Glutamate		 (( (Vmax * S) ) / (S + Km)) buffer: 0.2 mM NADH, 2 mM EDTA, 50 mM Hepes-KOH, 5 U lactate dehydrogenase
PH: 7.5
Temperature: 25
uniprot:P34106 [Pyridoxal phosphate]

2_Oxoglutarate + L_Alanine -> L_Glutamate + Pyruvate		 (( (Vmax * S) ) / (S + Km)) buffer: 0.2 mM NADH, 2 mM EDTA, 50 mM Hepes-KOH, 5 U lactate dehydrogenase
PH: 7.5
Temperature: 25
uniprot:Q9LR30 [alanine transaminase(Enzyme) wildtype GGT1]

Pyruvate + L_Glutamate -> 2_Oxoglutarate + L_Alanine		 (( (Vmax * A) ) / (Km + A)) buffer: 60 mM potassium phosphate, 0.17 mM NADH, 2.2 units/ml bovine liver glutamic dehydrogenase
PH: 7.5
Temperature: 25
- [alanine transaminase(Enzyme) wildtype GGT]

2_Oxoglutarate + L_Alanine -> L_Glutamate + Pyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 70 mM HEPES-NaOH, 0.17 mM NADH, 5 units/ml rabbit muscle lactate dehydrogenase
PH: 7
Temperature: 25
- [alanine transaminase(Enzyme) wildtype GGT]

L_Alanine + 2_Oxoglutarate -> Pyruvate + L_Glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 70 mM HEPES-NaOH, 0.17 mM NADH, 5 units/ml rabbit muscle lactate dehydrogenase
PH: 7
Temperature: 25
- [alanine transaminase(Enzyme) wildtype GGT]

L_Glutamate + Pyruvate -> 2_Oxoglutarate + L_Alanine		 (( (Vmax * A) ) / (Km + A)) buffer: 60 mM potassium phosphate, 0.17 mM NADH, 2.2 units/ml bovine liver glutamic dehydrogenase
PH: 7.5
Temperature: 25
uniprot:Q9LR30 [alanine transaminase(Enzyme) wildtype GGT1]

2_Oxoglutarate + L_Alanine -> L_Glutamate + Pyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 70 mM HEPES-NaOH, 0.17 mM NADH, 5 units/ml rabbit muscle lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:Q9LR30 [alanine transaminase(Enzyme) wildtype GGT1]

L_Alanine + 2_Oxoglutarate -> L_Glutamate + Pyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 70 mM HEPES-NaOH, 0.17 mM NADH, 5 units/ml rabbit muscle lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:Q9S7E9 [alanine transaminase(Enzyme) wildtype GGT2]

L_Alanine + 2_Oxoglutarate -> L_Glutamate + Pyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 70 mM HEPES-NaOH, 0.17 mM NADH, 5 units/ml rabbit muscle lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:Q9S7E9 [alanine transaminase(Enzyme) wildtype GGT2]

L_Alanine + 2_Oxoglutarate -> L_Glutamate + Pyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 70 mM HEPES-NaOH, 0.17 mM NADH, 5 units/ml rabbit muscle lactate dehydrogenase
PH: 7
Temperature: 25
uniprot:Q9S7E9 [alanine transaminase(Enzyme) wildtype GGT2]

L_Glutamate + Pyruvate -> 2_Oxoglutarate + L_Alanine		 (( (Vmax * A) ) / (Km + A)) buffer: 60 mM potassium phosphate, 0.17 mM NADH, 2.2 units/ml bovine liver glutamic dehydrogenase
PH: 7.5
Temperature: 25
- [alanine transaminase(Enzyme) wildtype]

2_Oxoglutarate + L_Alanine -> L_Glutamate + Pyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 0.11 mM Pyridoxalphosphate, 0.15 mM NADH, 1.0 IU lactate dehydrogenase
PH: 7.2
Temperature: 37
- [alanine transaminase(Enzyme) wildtype]

L_Alanine + 2_Oxoglutarate -> Pyruvate + L_Glutamate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 0.11 mM Pyridoxalphosphate, 0.15 mM NADH, 1.0 IU lactate dehydrogenase
PH: 7.2
Temperature: 37
uniprot:P33447 [alanine transaminase(Enzyme) wildtype]

L_Alanine + 2_Oxoglutarate -> Pyruvate + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 150 mM triethanolamine, 1 mM DTT, 1 mM EDTA
PH: 7.5
Temperature: 37
uniprot:P33447 [alanine transaminase(Enzyme) mutant N17S]

L_Alanine + 2_Oxoglutarate -> L_Glutamate + Pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 150 mM triethanolamine, 1 mM DTT, 1 mM EDTA
PH: 7.5
Temperature: 37
uniprot:P33447 [alanine transaminase(Enzyme) wildtype]

2_Oxoglutarate + L_Alanine -> Pyruvate + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 150 mM triethanolamine, 1 mM DTT, 1 mM EDTA
PH: 7.5
Temperature: 37
uniprot:P33447 [alanine transaminase(Enzyme) mutant N17S]

2_Oxoglutarate + L_Alanine -> Pyruvate + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 150 mM triethanolamine, 1 mM DTT, 1 mM EDTA
PH: 7.5
Temperature: 37
uniprot:P33447 [alanine transaminase(Enzyme) mutant R20A]

2_Oxoglutarate + L_Alanine -> Pyruvate + L_Glutamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 150 mM triethanolamine, 1 mM DTT, 1 mM EDTA
PH: 7.5
Temperature: 37
uniprot:P33447 [alanine transaminase(Enzyme) mutant R20A]

L_Alanine + 2_Oxoglutarate -> L_Glutamate + Pyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 150 mM triethanolamine, 1 mM DTT, 1 mM EDTA
PH: 7.5
Temperature: 37

Pathways

pathway id name
BioCyc:META_ALANINE-SYN2-PWY L-alanine biosynthesis II
BioCyc:MTBH37RV_PWY-5345 superpathway of L-methionine biosynthesis (by sulfhydrylation)
BioCyc:MTBH37RV_PWY-3001 superpathway of L-isoleucine biosynthesis I
BioCyc:MOUSE_ALANINE-DEG3-PWY alanine degradation III
BioCyc:META_PWY-181 photorespiration
BioCyc:YEAST_ALANINE-DEG3-PWY alanine degradation III
BioCyc:YEAST_ALANINE-SYN2-PWY alanine biosynthesis
BioCyc:MTBH37RV_ASPARTATESYN-PWY L-aspartate biosynthesis
BioCyc:MTBH37RV_ASPARTATE-DEG1-PWY L-aspartate degradation I
BioCyc:MTBH37RV_THRESYN-PWY superpathway of L-threonine biosynthesis
BioCyc:ECOL316407_PWY0-1061 superpathway of alanine biosynthesis
BioCyc:HUMAN_ALANINE-DEG3-PWY alanine biosynthesis/degradation
BioCyc:META_PWY0-1061 superpathway of L-alanine biosynthesis
BioCyc:META_ALANINE-DEG3-PWY L-alanine degradation III
BioCyc:ECO_PWY0-1061 superpathway of L-alanine biosynthesis
BioCyc:MTBH37RV_PHESYN L-phenylalanine biosynthesis I
BioCyc:META_PWY-7115 C4 photosynthetic carbon assimilation cycle, NAD-ME type
BioCyc:ECO_ALANINE-SYN2-PWY L-alanine biosynthesis II
BioCyc:MTBCDC1551_ALANINE-SYN2-PWY L-alanine biosynthesis II
BioCyc:MTBCDC1551_ALANINE-DEG3-PWY L-alanine degradation III
BioCyc:MOUSE_ALANINE-SYN2-PWY alanine biosynthesis II
BioCyc:ECOL316407_ALANINE-SYN2-PWY alanine biosynthesis II
PathBank:SMP0000810 L-Alanine Metabolism
PlantCyc:ARA_GLYSYN-ALA-PWY glycine biosynthesis III
PlantCyc:ARA_GLYSYN2-PWY glycine biosynthesis
PlantCyc:PLANT_ALANINE-DEG3-PWY L-alanine degradation III
PlantCyc:PLANT_ALANINE-SYN2-PWY L-alanine biosynthesis II
PlantCyc:ARA_PWY-181 photorespiration
PlantCyc:ARA_ALANINE-SYN2-PWY L-alanine biosynthesis II
PlantCyc:ARA_ALANINE-DEG3-PWY L-alanine degradation III
PlantCyc:PLANT_PWY-181 photorespiration
PlantCyc:ARA_ALACAT2-PWY L-alanine degradation II (to D-lactate)
PlantCyc:PLANT_PWY-7115 C4 photosynthetic carbon assimilation cycle, NAD-ME type
PlantCyc:PLANT_GLYSYN2-PWY glycine biosynthesis
Reactome:R-DRE-8964540 Alanine metabolism
Reactome:R-SPO-8964540 Alanine metabolism
Reactome:R-DDI-8964540 Alanine metabolism
Reactome:R-MMU-8964540 Alanine metabolism
Reactome:R-HSA-8964540 Alanine metabolism
Reactome:R-XTR-8964540 Alanine metabolism
Reactome:R-RNO-8964540 Alanine metabolism
Reactome:R-SCE-8964540 Alanine metabolism
Reactome:R-DRE-71291 Amino acid and derivative metabolism
Reactome:R-DDI-70268 Pyruvate metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-SCE-71291 Amino acid and derivative metabolism
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SPO-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-DRE-70268 Pyruvate metabolism
Reactome:R-DDI-71291 Amino acid and derivative metabolism
Reactome:R-HSA-70268 Pyruvate metabolism
Reactome:R-MMU-70268 Pyruvate metabolism
Reactome:R-RNO-70268 Pyruvate metabolism
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-70268 Pyruvate metabolism
Reactome:R-SPO-71291 Amino acid and derivative metabolism
Reactome:R-XTR-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-XTR-71291 Amino acid and derivative metabolism
Reactome:R-DRE-1430728 Metabolism
Reactome:R-DRE-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-HSA-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-SCE-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-XTR-1430728 Metabolism
Reactome:R-XTR-70268 Pyruvate metabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-HSA-1430728 Metabolism
Reactome:R-SPO-70268 Pyruvate metabolism
WikiPathways:WP4595 Urea cycle and associated pathways
WikiPathways:WP4583 Biomarkers for urea cycle disorders
WikiPathways:WP5355 Metabolic Epileptic Disorders
WikiPathways:WP4236 Krebs cycle disorders
WikiPathways:WP5220 Metabolic reprogramming in pancreatic cancer
PathBank:SMP0000055 Alanine Metabolism
PathBank:SMP0000059 Urea Cycle
PathBank:SMP0000339 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0000001 Citrullinemia Type I
PathBank:SMP0087169 Glutamate Metabolism
PathBank:SMP0087221 Glucose-Alanine Cycle
PathBank:SMP0000127 Glucose-Alanine Cycle
PathBank:SMP0000385 Homocarnosinosis
PathBank:SMP0000003 Argininosuccinic Aciduria
PathBank:SMP0000136 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0000567 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0002301 L-Alanine Metabolism
PathBank:SMP0087164 Alanine Metabolism
PathBank:SMP0087224 Urea Cycle
PathBank:SMP0000352 Primary Hyperoxaluria Type I
PathBank:SMP0000205 Ornithine Transcarbamylase Deficiency (OTC Deficiency)
PathBank:SMP0000072 Glutamate Metabolism
PathBank:SMP0000350 Pyruvate Carboxylase Deficiency
PathBank:SMP0000243 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000313 Lactic Acidemia
PathBank:SMP0000357 Argininemia
PathBank:SMP0000002 Carbamoyl Phosphate Synthetase Deficiency
PathBank:SMP0002298 Glutaminolysis and Cancer
PathBank:SMP0002415 Alanine Metabolism