EC: 2.3.1.8

phosphate acetyltransferase (acetyl-CoA:phosphate acetyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:P38503 [phosphate acetyltransferase(Enzyme) mutant R87Q]

Coenzyme_A + Acetyl_phosphate -> Phosphate + Acetyl_CoA		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 20 mM NH4Cl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7.2
Temperature: 23
uniprot:P38503 [phosphate acetyltransferase(Enzyme) mutant R310Q]

Acetyl_phosphate + Coenzyme_A -> Phosphate + Acetyl_CoA		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 20 mM NH4Cl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7.2
Temperature: 23
uniprot:P38503 [phosphate acetyltransferase(Enzyme) mutant C159S]

Acetyl_phosphate + Coenzyme_A -> Acetyl_CoA + Phosphate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 20 mM NH4Cl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7.2
Temperature: 23
uniprot:P38503 [phosphate acetyltransferase(Enzyme) mutant R133Q]

Acetyl_phosphate + Coenzyme_A -> Acetyl_CoA + Phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 20 mM NH4Cl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7.2
Temperature: 23
uniprot:P38503 [phosphate acetyltransferase(Enzyme) mutant C277A, C312A, C325A]

Acetyl_phosphate + Coenzyme_A -> Phosphate + Acetyl_CoA		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 20 mM NH4Cl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7.2
Temperature: 23
uniprot:P38503 [phosphate acetyltransferase(Enzyme) mutant C325A]

Acetyl_phosphate + Coenzyme_A -> Acetyl_CoA + Phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 20 mM NH4Cl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7.2
Temperature: 23
uniprot:P38503 [phosphate acetyltransferase(Enzyme) mutant R28Q]

Coenzyme_A + Acetyl_phosphate -> Acetyl_CoA + Phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 20 mM NH4Cl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7.2
Temperature: 23
uniprot:P38503 [phosphate acetyltransferase(Enzyme) mutant R287Q]

Acetyl_phosphate + Coenzyme_A -> Acetyl_CoA + Phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 20 mM NH4Cl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7.2
Temperature: 23
uniprot:P38503 [phosphate acetyltransferase(Enzyme) mutant C159A, C277A, C312A, C325A]

Coenzyme_A + Acetyl_phosphate -> Acetyl_CoA + Phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 20 mM NH4Cl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7.2
Temperature: 23
uniprot:P38503 [phosphate acetyltransferase(Enzyme) mutant R310Q]

Acetyl_phosphate + Coenzyme_A -> Acetyl_CoA + Phosphate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 20 mM NH4Cl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7.2
Temperature: 23
uniprot:P38503 [phosphate acetyltransferase(Enzyme) mutant C159S]

Coenzyme_A + Acetyl_phosphate -> Phosphate + Acetyl_CoA		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 20 mM NH4Cl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7.2
Temperature: 23
uniprot:P38503 [phosphate acetyltransferase(Enzyme) wildtype]

Acetyl_phosphate + Coenzyme_A -> Phosphate + Acetyl_CoA		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 20 mM NH4Cl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7.2
Temperature: 23
uniprot:P38503 [phosphate acetyltransferase(Enzyme) mutant C277A]

Acetyl_phosphate + Coenzyme_A -> Acetyl_CoA + Phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 20 mM NH4Cl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7.2
Temperature: 23
uniprot:P38503 [phosphate acetyltransferase(Enzyme) wildtype]

Acetyl_phosphate + Coenzyme_A -> Phosphate + Acetyl_CoA		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 20 mM NH4Cl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7.2
Temperature: 23
uniprot:P38503 [phosphate acetyltransferase(Enzyme) mutant C159A]

Acetyl_phosphate + Coenzyme_A -> Phosphate + Acetyl_CoA		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 20 mM NH4Cl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7.2
Temperature: 23
uniprot:P38503 [phosphate acetyltransferase(Enzyme) mutant C159A]

Acetyl_phosphate + Coenzyme_A -> Phosphate + Acetyl_CoA		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 20 mM NH4Cl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7.2
Temperature: 23
uniprot:P38503 [phosphate acetyltransferase(Enzyme) mutant C312A]

Acetyl_phosphate + Coenzyme_A -> Phosphate + Acetyl_CoA		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 20 mM NH4Cl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7.2
Temperature: 23
uniprot:P38503 [phosphate acetyltransferase(Enzyme) wildtype]

Acetyl_phosphate + Coenzyme_A -> Acetyl_CoA + Phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 20 mM NH4Cl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7.2
Temperature: 23
uniprot:P38503 [phosphate acetyltransferase(Enzyme) wildtype]

Acetyl_phosphate + Coenzyme_A -> Acetyl_CoA + Phosphate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 20 mM NH4Cl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7.2
Temperature: 23
uniprot:P38503 [phosphate acetyltransferase(Enzyme) mutant C277A]

Acetyl_phosphate + Coenzyme_A -> Acetyl_CoA + Phosphate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 20 mM NH4Cl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7.2
Temperature: 23
uniprot:P38503 [phosphate acetyltransferase(Enzyme) mutant R28Q]

Coenzyme_A + Acetyl_phosphate -> Acetyl_CoA + Phosphate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 20 mM NH4Cl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7.2
Temperature: 23
uniprot:P38503 [phosphate acetyltransferase(Enzyme) mutant C325A]

Acetyl_phosphate + Coenzyme_A -> Phosphate + Acetyl_CoA		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 20 mM NH4Cl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7.2
Temperature: 23
uniprot:P38503 [phosphate acetyltransferase(Enzyme) mutant R133Q]

Acetyl_phosphate + Coenzyme_A -> Acetyl_CoA + Phosphate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 20 mM NH4Cl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7.2
Temperature: 23
uniprot:P38503 [phosphate acetyltransferase(Enzyme) mutant C277A, C312A, C325A]

Coenzyme_A + Acetyl_phosphate -> Phosphate + Acetyl_CoA		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 20 mM NH4Cl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7.2
Temperature: 23
uniprot:P38503 [phosphate acetyltransferase(Enzyme) mutant C159A, C277A, C312A, C325A]

Acetyl_phosphate + Coenzyme_A -> Acetyl_CoA + Phosphate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 20 mM NH4Cl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7.2
Temperature: 23
uniprot:P38503 [phosphate acetyltransferase(Enzyme) mutant R287Q]

Coenzyme_A + Acetyl_phosphate -> Phosphate + Acetyl_CoA		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 20 mM NH4Cl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7.2
Temperature: 23
uniprot:P38503 [phosphate acetyltransferase(Enzyme) mutant C312A]

Acetyl_phosphate + Coenzyme_A -> Phosphate + Acetyl_CoA		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 20 mM NH4Cl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7.2
Temperature: 23
uniprot:P38503 [phosphate acetyltransferase(Enzyme) mutant R87Q]

Coenzyme_A + Acetyl_phosphate -> Acetyl_CoA + Phosphate		 (( (( (kcat * E) ) * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 20 mM NH4Cl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7.2
Temperature: 23
uniprot:P0A9M8 [phosphate acetyltransferase(Enzyme) mutant Pta-F1 (last 326 amino acids), N-terminal His-tag]

Phosphate + Acetyl_CoA -> Acetyl_phosphate + Coenzyme_A		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl, 20 mM KCl, 0.1 mM 5',5-Dithiobis(2-nitrobenzoic acid)
PH: 7
Temperature: 30
uniprot:P0A9M8 [phosphate acetyltransferase(Enzyme) mutant Pta-F3 (last 470 amino acids), N-terminal His-tag]

Phosphate + Acetyl_CoA -> Coenzyme_A + Acetyl_phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl, 20 mM KCl, 0.1 mM 5',5-Dithiobis(2-nitrobenzoic acid)
PH: 7
Temperature: 30
uniprot:P0A9M8 [phosphate acetyltransferase(Enzyme) mutant Pta-F2 (last 352 amino acids), N-terminal His-tag]

Coenzyme_A + Acetyl_phosphate -> Phosphate + Acetyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7
Temperature: 30
uniprot:P0A9M8 [phosphate acetyltransferase(Enzyme) mutant Pta-F1 (last 326 amino acids), N-terminal His-tag]

Coenzyme_A + Acetyl_phosphate -> Phosphate + Acetyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7
Temperature: 30
uniprot:P0A9M8 [phosphate acetyltransferase(Enzyme) mutant Pta-F1 (last 326 amino acids), N-terminal His-tag]

Acetyl_phosphate + Coenzyme_A -> Phosphate + Acetyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7
Temperature: 30
uniprot:P0A9M8 [phosphate acetyltransferase(Enzyme) wildtype N-terminal His-tag]

Coenzyme_A + Acetyl_phosphate -> Phosphate + Acetyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7
Temperature: 30
uniprot:P0A9M8 [phosphate acetyltransferase(Enzyme) wildtype N-terminal His-tag]

Phosphate + Acetyl_CoA -> Coenzyme_A + Acetyl_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 20 mM KCl, 0.1 mM 5',5-Dithiobis(2-nitrobenzoic acid)
PH: 7
Temperature: 30
uniprot:P0A9M8 [phosphate acetyltransferase(Enzyme) wildtype N-terminal His-tag]

Coenzyme_A + Acetyl_phosphate -> Phosphate + Acetyl_CoA		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7
Temperature: 30
uniprot:P0A9M8 [phosphate acetyltransferase(Enzyme) wildtype N-terminal His-tag]

Acetyl_CoA + Phosphate -> Coenzyme_A + Acetyl_phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl, 20 mM KCl, 0.1 mM 5',5-Dithiobis(2-nitrobenzoic acid)
PH: 7
Temperature: 30
uniprot:P0A9M8 [phosphate acetyltransferase(Enzyme) mutant Pta-F2 (last 352 amino acids), N-terminal His-tag]

Acetyl_CoA + Phosphate -> Coenzyme_A + Acetyl_phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl, 20 mM KCl, 0.1 mM 5',5-Dithiobis(2-nitrobenzoic acid)
PH: 7
Temperature: 30
uniprot:P0A9M8 [phosphate acetyltransferase(Enzyme) mutant Pta-F3 (last 470 amino acids), N-terminal His-tag]

Coenzyme_A + Acetyl_phosphate -> Acetyl_CoA + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7
Temperature: 30
uniprot:P0A9M8 [phosphate acetyltransferase(Enzyme) mutant Pta-F2 (last 352 amino acids), N-terminal His-tag]

Coenzyme_A + Acetyl_phosphate -> Acetyl_CoA + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7
Temperature: 30
uniprot:P0A9M8 [phosphate acetyltransferase(Enzyme) mutant Pta-F3 (last 470 amino acids), N-terminal His-tag]

Coenzyme_A + Acetyl_phosphate -> Phosphate + Acetyl_CoA		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 20 mM KCl, 2 mM Dithiothreitol
PH: 7
Temperature: 30
uniprot:P0A9M8 [phosphate acetyltransferase(Enzyme) mutant Pta-F2 (last 352 amino acids), N-terminal His-tag]

Phosphate + Acetyl_CoA -> Acetyl_phosphate + Coenzyme_A		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 20 mM KCl, 0.1 mM 5',5-Dithiobis(2-nitrobenzoic acid)
PH: 7
Temperature: 30
uniprot:P0A9M8 [phosphate acetyltransferase(Enzyme) mutant Pta-F3 (last 470 amino acids), N-terminal His-tag]

Acetyl_CoA + Phosphate -> Acetyl_phosphate + Coenzyme_A		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 20 mM KCl, 0.1 mM 5',5-Dithiobis(2-nitrobenzoic acid)
PH: 7
Temperature: 30
uniprot:P0A9M8 [phosphate acetyltransferase(Enzyme) mutant Pta-F1 (last 326 amino acids), N-terminal His-tag]

Phosphate + Acetyl_CoA -> Acetyl_phosphate + Coenzyme_A		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 20 mM KCl, 0.1 mM 5',5-Dithiobis(2-nitrobenzoic acid)
PH: 7
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-1281 sulfoacetaldehyde degradation I
BioCyc:META_PWY-5485 pyruvate fermentation to acetate IV
BioCyc:META_P142-PWY pyruvate fermentation to acetate I
BioCyc:META_P441-PWY superpathway of N-acetylneuraminate degradation
BioCyc:META_PWY-5437 L-threonine degradation I
BioCyc:VCHO_PWY0-1312 acetate formation from acetyl-CoA I
BioCyc:VCHO_P41-PWY pyruvate fermentation to acetate and lactate I
BioCyc:MTBH37RV_PWY-5482 pyruvate fermentation to acetate II
BioCyc:BSUB_PWY-5485 pyruvate fermentation to acetate IV
BioCyc:ECOL316407_PWY0-1312 acetate formation from acetyl-CoA I
BioCyc:META_PWY-5482 pyruvate fermentation to acetate II
BioCyc:META_PWY-6604 superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation
BioCyc:META_THREOCAT-PWY superpathway of L-threonine metabolism
BioCyc:ECO_THREOCAT-PWY superpathway of L-threonine metabolism
BioCyc:ECO_ACETATEUTIL-PWY superpathway of acetate utilization and formation
BioCyc:MTBH37RV_PWY0-1312 acetate and ATP formation from acetyl-CoA I
BioCyc:MTBH37RV_ACETATEUTIL-PWY superpathway of acetate utilization and formation
BioCyc:BSUB_PWY0-1312 acetate and ATP formation from acetyl-CoA I
BioCyc:BSUB_FERMENTATION-PWY mixed acid fermentation
BioCyc:BSUB_PWY-5676 acetyl-CoA fermentation to butanoate II
BioCyc:CORYNE_PWY0-1312 acetate formation from acetyl-CoA I
BioCyc:META_P41-PWY pyruvate fermentation to acetate and lactate I
BioCyc:META_PWY0-1477 ethanolamine utilization
BioCyc:META_PWY-5327 superpathway of L-lysine degradation
BioCyc:META_PWY0-1312 acetate and ATP formation from acetyl-CoA I
BioCyc:META_ACETATEUTIL-PWY superpathway of acetate utilization and formation
BioCyc:META_PWY-1541 superpathway of taurine degradation
BioCyc:ECO_PWY0-1312 acetate and ATP formation from acetyl-CoA I
BioCyc:VCHO_PWY-5437 L-threonine degradation I
BioCyc:VCHO_PWY-5482 pyruvate fermentation to acetate II
BioCyc:VCHO_P441-PWY superpathway of N-acetylneuraminate degradation
BioCyc:MTBH37RV_FERMENTATION-PWY mixed acid fermentation
BioCyc:MTBCDC1551_PWY-5676 acetyl-CoA fermentation to butanoate II
BioCyc:MTBCDC1551_PWY-5485 pyruvate fermentation to acetate IV
BioCyc:MTBCDC1551_PWY-5482 pyruvate fermentation to acetate II
BioCyc:ECOL316407_FERMENTATION-PWY mixed acid fermentation
BioCyc:ECOL316407_PWY-5437 threonine degradation I
BioCyc:CORYNE_FERMENTATION-PWY mixed acid fermentation
BioCyc:META_PWY-5100 pyruvate fermentation to acetate and lactate II
BioCyc:META_PWY-6590 superpathway of Clostridium acetobutylicum acidogenic fermentation
BioCyc:META_FERMENTATION-PWY mixed acid fermentation
BioCyc:ECO_PWY0-1477 ethanolamine utilization
BioCyc:ECO_FERMENTATION-PWY mixed acid fermentation
BioCyc:ECO_PWY-5437 L-threonine degradation I
BioCyc:VCHO_PWY-5485 pyruvate fermentation to acetate IV
BioCyc:VCHO_ACETATEUTIL-PWY superpathway of acetate utilization and formation
BioCyc:MTBH37RV_PWY-5485 pyruvate fermentation to acetate IV
BioCyc:MTBCDC1551_FERMENTATION-PWY mixed acid fermentation
BioCyc:MTBCDC1551_PWY0-1312 acetate formation from acetyl-CoA I
BioCyc:MTBCDC1551_ACETATEUTIL-PWY superpathway of acetate utilization and formation
BioCyc:BSUB_ACETATEUTIL-PWY superpathway of acetate utilization and formation
BioCyc:ECOL316407_ACETATEUTIL-PWY superpathway of acetate utilization and formation
BioCyc:ECOL316407_THREOCAT-PWY superpathway of threonine metabolism
PathBank:SMP0000957 Propanoate Metabolism
PathBank:SMP0002102 Acetate Metabolism