EC: 1.1.1.357

3alpha-hydroxysteroid 3-dehydrogenase (3alpha-hydroxysteroid:NAD(P)+ 3-oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:P52895 [3alpha-hydroxysteroid 3-dehydrogenase(Enzyme) wildtype]

NAD+ + Testosterone -> Androst_4_ene_3,17_dione + NADH + H+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM potassium phosphate, 4% acetonitrile
PH: 7
Temperature: 37
uniprot:P52895 [3alpha-hydroxysteroid 3-dehydrogenase(Enzyme) wildtype]

NAD+ + 20alpha_Hydroxy_4_pregnen_3_one -> Progesterone + H+ + NADH		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM potassium phosphate, 4% acetonitrile
PH: 7
Temperature: 37
uniprot:P52895 [3alpha-hydroxysteroid 3-dehydrogenase(Enzyme) wildtype]

Estradiol_17beta + NAD+ -> H+ + NADH + Estrone		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM potassium phosphate, 4% acetonitrile
PH: 7
Temperature: 37
uniprot:P52895 [3alpha-hydroxysteroid 3-dehydrogenase(Enzyme) wildtype]

NAD+ + Epiandrosterone -> NADH + H+ + 5alpha_Androstane_3,17_dione		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM potassium phosphate, 4% acetonitrile
PH: 7
Temperature: 37
uniprot:P52895 [3alpha-hydroxysteroid 3-dehydrogenase(Enzyme) wildtype]

NAD+ + 3alpha_Hydroxy_5beta_androstan_17_one -> H+ + NADH + 5beta_Androstane_3,17_dione		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM potassium phosphate, 4% acetonitrile
PH: 7
Temperature: 37
uniprot:P52895 [3alpha-hydroxysteroid 3-dehydrogenase(Enzyme) wildtype]

5alpha_Androstane_3alpha_ol_17_one + NAD+ -> 5alpha_Androstane_3,17_dione + H+ + NADH		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM potassium phosphate, 4% acetonitrile
PH: 7
Temperature: 37
uniprot:P52895 [3alpha-hydroxysteroid 3-dehydrogenase(Enzyme) wildtype]

NAD+ + 5alpha_Androstane_17beta_ol_3_one -> 5alpha_Androstane_3,17_dione + H+ + NADH		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM potassium phosphate, 4% acetonitrile
PH: 7
Temperature: 37
uniprot:P52895 [3alpha-hydroxysteroid 3-dehydrogenase(Enzyme) wildtype]

NAD+ + 3beta_Hydroxy_5beta_androstan_17_one -> NADH + 5beta_Androstane_3,17_dione + H+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM potassium phosphate, 4% acetonitrile
PH: 7
Temperature: 37
uniprot:P52895 [3alpha-hydroxysteroid 3-dehydrogenase(Enzyme) mutant AKR1C2 Ile79Val]

NAD+ + Androstanediol -> H+ + NADH + 5alpha_Androstane_17beta_ol_3_one		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Kip
PH: 7.4
Temperature: 37
uniprot:P52895 [3alpha-hydroxysteroid 3-dehydrogenase(Enzyme) wildtype AKR1C2]

Androstanediol + NAD+ -> NADH + H+ + 5alpha_Androstane_17beta_ol_3_one		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Kip
PH: 7.4
Temperature: 37
uniprot:P52895 [3alpha-hydroxysteroid 3-dehydrogenase(Enzyme) mutant AKR1C2 Asn300Thr]

NAD+ + Androstanediol -> H+ + NADH + 5alpha_Androstane_17beta_ol_3_one		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Kip
PH: 7.4
Temperature: 37
uniprot:P52895 [3alpha-hydroxysteroid 3-dehydrogenase(Enzyme) mutant AKR1C2 His90Gln]

Androstanediol + NAD+ -> H+ + 5alpha_Androstane_17beta_ol_3_one + NADH		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Kip
PH: 7.4
Temperature: 37
uniprot:P52895 [3alpha-hydroxysteroid 3-dehydrogenase(Enzyme) mutant AKR1C2 His90Gln]

NADPH + 5alpha_Pregnane_3,20_dione + H+ -> 3alpha_Hydroxy_5alpha_pregnan_20_one + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Kip
PH: 7.4
Temperature: 37
uniprot:P52895 [3alpha-hydroxysteroid 3-dehydrogenase(Enzyme) mutant AKR1C2 Ile79Val]

NADPH + 5alpha_Pregnane_3,20_dione + H+ -> NADP+ + 3alpha_Hydroxy_5alpha_pregnan_20_one		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Kip
PH: 7.4
Temperature: 37
uniprot:P52895 [3alpha-hydroxysteroid 3-dehydrogenase(Enzyme) wildtype AKR1C2]

NADPH + 5alpha_Pregnane_3,20_dione + H+ -> NADP+ + 3alpha_Hydroxy_5alpha_pregnan_20_one		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Kip
PH: 7.4
Temperature: 37
uniprot:P52895 [3alpha-hydroxysteroid 3-dehydrogenase(Enzyme) mutant AKR1C2 Asn300Thr]

H+ + NADPH + 5alpha_Pregnane_3,20_dione -> NADP+ + 3alpha_Hydroxy_5alpha_pregnan_20_one		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Kip
PH: 7.4
Temperature: 37
uniprot:P52895 [3alpha-hydroxysteroid 3-dehydrogenase(Enzyme) wildtype AKR1C2]

Androstanediol + NAD+ -> NADH + H+ + 5alpha_Androstane_17beta_ol_3_one		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Kip
PH: 7.4
Temperature: 37
uniprot:P17516 [3alpha-hydroxysteroid 3-dehydrogenase(Enzyme) wildtype AKR1C4]

NADPH + 5alpha_Pregnane_3,20_dione + H+ -> 3alpha_Hydroxy_5alpha_pregnan_20_one + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Kip
PH: 7.4
Temperature: 37
uniprot:P17516 [3alpha-hydroxysteroid 3-dehydrogenase(Enzyme) mutant AKR1C4 V29_K84del]

H+ + 5alpha_Pregnane_3,20_dione + NADPH -> NADP+ + 3alpha_Hydroxy_5alpha_pregnan_20_one		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Kip
PH: 7.4
Temperature: 37
uniprot:P52895 [Fluoxetine]

NADP+ + 3alpha_Hydroxy_5alpha_pregnan_20_one -> 5alpha_Pregnane_3,20_dione + H+ + NADPH		 (( (Vmax * A) ) / (Km_A + A)) buffer: 100 mM sodium phosphate
PH: 8.9
Temperature: 37
uniprot:P52895 [Imipramine]

3alpha_Hydroxy_5alpha_pregnan_20_one + NADP+ -> 5alpha_Pregnane_3,20_dione + NADPH + H+		 (( (Vmax * A) ) / (Km_A + A)) buffer: 100 mM sodium phosphate
PH: 8.9
Temperature: 37
uniprot:P52895 [Imipramine]

NADPH + H+ + 5alpha_Pregnane_3,20_dione -> NADP+ + 3alpha_Hydroxy_5alpha_pregnan_20_one		 (( (Vmax * A) ) / (Km_A + A)) buffer: 100 mM sodium phosphate
PH: 7.3
Temperature: 37
uniprot:P52895 [Paroxetine]

3alpha_Hydroxy_5alpha_pregnan_20_one + NADP+ -> H+ + NADPH + 5alpha_Pregnane_3,20_dione		 (( (Vmax * A) ) / (Km_A + A)) buffer: 100 mM sodium phosphate
PH: 8.9
Temperature: 37
uniprot:P52895 [Sertraline]

3alpha_Hydroxy_5alpha_pregnan_20_one + NADP+ -> NADPH + 5alpha_Pregnane_3,20_dione + H+		 (( (Vmax * A) ) / (Km_A + A)) buffer: 100 mM sodium phosphate
PH: 8.9
Temperature: 37
uniprot:P52895 [Paroxetine]

5alpha_Pregnane_3,20_dione + NADPH + H+ -> 3alpha_Hydroxy_5alpha_pregnan_20_one + NADP+		 (( (Vmax * A) ) / (Km_A + A)) buffer: 100 mM sodium phosphate
PH: 7.3
Temperature: 37
uniprot:P52895 [Sertraline]

NADPH + H+ + 5alpha_Pregnane_3,20_dione -> 3alpha_Hydroxy_5alpha_pregnan_20_one + NADP+		 (( (Vmax * A) ) / (Km_A + A)) buffer: 100 mM sodium phosphate
PH: 7.3
Temperature: 37
uniprot:P52895 [3alpha-hydroxysteroid 3-dehydrogenase(Enzyme) wildtype]

NADP+ + 3alpha_Hydroxy_5alpha_pregnan_20_one -> 5alpha_Pregnane_3,20_dione + H+ + NADPH		 (( (Vmax * A) ) / (Km_A + A)) buffer: 100 mM sodium phosphate
PH: 8.9
Temperature: 37
uniprot:P52895 [Fluoxetine]

NADPH + 5alpha_Pregnane_3,20_dione + H+ -> 3alpha_Hydroxy_5alpha_pregnan_20_one + NADP+		 (( (Vmax * A) ) / (Km_A + A)) buffer: 100 mM sodium phosphate
PH: 7.3
Temperature: 37
uniprot:P52895 [3alpha-hydroxysteroid 3-dehydrogenase(Enzyme) wildtype]

NADPH + 5alpha_Pregnane_3,20_dione + H+ -> 3alpha_Hydroxy_5alpha_pregnan_20_one + NADP+		 (( (Vmax * A) ) / (Km_A + A)) buffer: 100 mM sodium phosphate
PH: 7.3
Temperature: 37
uniprot:P42330 [Fluoxetine]

5alpha_Androstane_17beta_ol_3_one + NADPH + H+ -> Androstanediol + NADP+		 (( (Vmax * A) ) / (Km_A + A)) buffer: 100 mM sodium phosphate
PH: 7.3
Temperature: 37
uniprot:P42330 [Imipramine]

NADPH + H+ + 5alpha_Androstane_17beta_ol_3_one -> NADP+ + Androstanediol		 (( (Vmax * A) ) / (Km_A + A)) buffer: 100 mM sodium phosphate
PH: 7.3
Temperature: 37
uniprot:P42330 [Paroxetine]

H+ + 5alpha_Androstane_17beta_ol_3_one + NADPH -> Androstanediol + NADP+		 (( (Vmax * A) ) / (Km_A + A)) buffer: 100 mM sodium phosphate
PH: 7.3
Temperature: 37
uniprot:P42330 [3alpha-hydroxysteroid 3-dehydrogenase(Enzyme) wildtype]

NADPH + 5alpha_Androstane_17beta_ol_3_one + H+ -> NADP+ + Androstanediol		 (( (Vmax * A) ) / (Km_A + A)) buffer: 100 mM sodium phosphate
PH: 7.3
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY-7455 allopregnanolone biosynthesis
BioCyc:HUMAN_PWY66-378 androgen biosynthesis
BioCyc:META_PWY66-378 androgen biosynthesis
BioCyc:META_PWY-7455 allopregnanolone biosynthesis
Reactome:R-HSA-9757110 Prednisone ADME
Reactome:R-HSA-9748784 Drug ADME
Reactome:R-HSA-5362517 Signaling by Retinoic Acid
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-8978868 Fatty acid metabolism
Reactome:R-HSA-2162123 Synthesis of Prostaglandins (PG) and Thromboxanes (TX)
Reactome:R-HSA-9006931 Signaling by Nuclear Receptors
Reactome:R-HSA-5365859 RA biosynthesis pathway
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-2142753 Arachidonate metabolism
Reactome:R-HSA-162582 Signaling Pathways
WikiPathways:WP5280 Glucocorticoid biosynthesis
WikiPathways:WP5176 Disorders of bile acid synthesis and biliary transport
WikiPathways:WP4524 Alternative pathway of fetal androgen synthesis
PathBank:SMP0030406 Androstenedione Metabolism
PathBank:SMP0000083 Acetylsalicylic Acid Action Pathway
PathBank:SMP0000087 Rofecoxib Action Pathway
PathBank:SMP0000094 Sulindac Action Pathway
PathBank:SMP0000373 Adrenal Hyperplasia Type 3 or Congenital Adrenal Hyperplasia Due to 21-Hydroxylase Deficiency
PathBank:SMP0000318 Congenital Bile Acid Synthesis Defect Type III
PathBank:SMP0000104 Indomethacin Action Pathway
PathBank:SMP0000120 Naproxen Action Pathway
PathBank:SMP0000106 Meloxicam Action Pathway
PathBank:SMP0000576 21-Hydroxylase Deficiency (CYP21)
PathBank:SMP0000693 Antrafenine Action Pathway
PathBank:SMP0000697 Flurbiprofen Action Pathway
PathBank:SMP0000701 Phenylbutazone Action Pathway
PathBank:SMP0000705 Tiaprofenic Acid Action Pathway
PathBank:SMP0000709 Salicylic Acid Action Pathway
PathBank:SMP0000718 3-beta-Hydroxysteroid Dehydrogenase Deficiency
PathBank:SMP0000035 Bile Acid Biosynthesis
PathBank:SMP0000075 Arachidonic Acid Metabolism
PathBank:SMP0000086 Ibuprofen Action Pathway
PathBank:SMP0000093 Diclofenac Action Pathway
PathBank:SMP0000098 Ketorolac Action Pathway
PathBank:SMP0000314 Congenital Bile Acid Synthesis Defect Type II
PathBank:SMP0000315 Cerebrotendinous Xanthomatosis (CTX)
PathBank:SMP0000102 Bromfenac Action Pathway
PathBank:SMP0000114 Nabumetone Action Pathway
PathBank:SMP0000575 11-beta-Hydroxylase Deficiency (CYP11B1)
PathBank:SMP0000692 Antipyrine Action Pathway
PathBank:SMP0000696 Fenoprofen Action Pathway
PathBank:SMP0000700 Lornoxicam Action Pathway
PathBank:SMP0000704 Tolmetin Action Pathway
PathBank:SMP0000708 Salicylate-Sodium Action Pathway
PathBank:SMP0000717 Apparent Mineralocorticoid Excess Syndrome
PathBank:SMP0000130 Steroidogenesis
PathBank:SMP0000077 Piroxicam Action Pathway
PathBank:SMP0000084 Etodolac Action Pathway
PathBank:SMP0000371 Congenital Lipoid Adrenal Hyperplasia (CLAH) or Lipoid CAH
PathBank:SMP0000317 Familial Hypercholanemia (FHCA)
PathBank:SMP0000109 Mefenamic Acid Action Pathway
PathBank:SMP0000289 Diflunisal Action Pathway
PathBank:SMP0000116 Valdecoxib Action Pathway
PathBank:SMP0000577 Corticosterone Methyl Oxidase I Deficiency (CMO I)
PathBank:SMP0000650 Doxorubicin Metabolism Pathway
PathBank:SMP0000694 Carprofen Action Pathway
PathBank:SMP0000698 Magnesium Salicylate Action Pathway
PathBank:SMP0000702 Nepafenac Action Pathway
PathBank:SMP0000706 Tenoxicam Action Pathway
PathBank:SMP0000710 Acetaminophen Action Pathway
PathBank:SMP0000353 Leukotriene C4 Synthesis Deficiency
PathBank:SMP0000085 Ketoprofen Action Pathway
PathBank:SMP0000096 Celecoxib Action Pathway
PathBank:SMP0000372 Adrenal Hyperplasia Type 5 or Congenital Adrenal Hyperplasia Due to 17 alpha-Hydroxylase Deficiency
PathBank:SMP0000316 Zellweger Syndrome
PathBank:SMP0000101 Suprofen Action Pathway
PathBank:SMP0000113 Oxaprozin Action Pathway
PathBank:SMP0000566 17-alpha-Hydroxylase Deficiency (CYP17)
PathBank:SMP0000578 Corticosterone Methyl Oxidase II Deficiency (CMO II)
PathBank:SMP0000695 Etoricoxib Action Pathway
PathBank:SMP0000699 Lumiracoxib Action Pathway
PathBank:SMP0000703 Trisalicylate-Choline Action Pathway
PathBank:SMP0000707 Salsalate Action Pathway
PathBank:SMP0000720 27-Hydroxylase Deficiency