EC: 1.1.1.3

homoserine dehydrogenase (L-homoserine:NAD(P)+ oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:O81852 [L-Threonine]

H+ + NADPH + L_Aspartate_4_semialdehyde -> L_Homoserine + NADP+		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Tris-HCl, 150 mM KCl
PH: 8
Temperature: 37
uniprot:O81852 [Threonine]

L_Aspartate_4_semialdehyde + H+ + NADPH -> NADP+ + L_Homoserine		 (( (( (kcat * E) ) * I) ) / (Km + I)) buffer: 50 mM Hepes, 150 mM KCl
PH: 8
Temperature: 30
uniprot:Q9SA18 [Threonine]

L_Aspartate_4_semialdehyde + H+ + NADPH -> L_Homoserine + NADP+		 (( (( (kcat * E) ) * I) ) / (Km + I)) buffer: 50 mM Hepes, 150 mM KCl
PH: 8
Temperature: 30
uniprot:Q9SA18 [Cysteine]

NADPH + L_Aspartate_4_semialdehyde + H+ -> L_Homoserine + NADP+		 (( (( (kcat * E) ) * I) ) / (Km + I)) buffer: 50 mM Hepes, 150 mM KCl
PH: 8
Temperature: 30
uniprot:O81852 [Cysteine]

NADPH + H+ + L_Aspartate_4_semialdehyde -> L_Homoserine + NADP+		 (( (( (kcat * E) ) * I) ) / (Km + I)) buffer: 50 mM Hepes, 150 mM KCl
PH: 8
Temperature: 30
uniprot:O81852 [homoserine dehydrogenase(Enzyme) wildtype HSDH II]

L_Aspartate_4_semialdehyde + NADPH + H+ -> L_Homoserine + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Hepes, 150 mM KCl
PH: 8
Temperature: 30
uniprot:Q9SA18 [homoserine dehydrogenase(Enzyme) wildtype HSDH I]

L_Aspartate_4_semialdehyde + NADPH + H+ -> L_Homoserine + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Hepes, 150 mM KCl
PH: 8
Temperature: 30
uniprot:O81852 [Alanine]

H+ + NADPH + L_Aspartate_4_semialdehyde -> L_Homoserine + NADP+		 (( (( (kcat * E) ) * I) ) / (Km + I)) buffer: 50 mM Hepes, 150 mM KCl
PH: 8
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY0-781 aspartate superpathway
BioCyc:MTBH37RV_PWY-5345 superpathway of L-methionine biosynthesis (by sulfhydrylation)
BioCyc:MTBH37RV_PWY-3001 superpathway of L-isoleucine biosynthesis I
BioCyc:BSUB_METSYN-PWY superpathway of L-homoserine and L-methionine biosynthesis
BioCyc:ECOL316407_P4-PWY superpathway of lysine, threonine and methionine biosynthesis I
BioCyc:ECOL316407_METSYN-PWY homoserine and methionine biosynthesis
BioCyc:ECOL316407_PWY0-781 aspartate superpathway
BioCyc:CORYNE_PWY0-781 aspartate superpathway
BioCyc:CORYNE_MET-SAM-PWY superpathway of S-adenosyl-L-methionine biosynthesis
BioCyc:META_HOMOSERSYN-PWY L-homoserine biosynthesis
BioCyc:ECO_P4-PWY superpathway of L-lysine, L-threonine and L-methionine biosynthesis I
BioCyc:MTBH37RV_HOMOSERSYN-PWY L-homoserine biosynthesis
BioCyc:MTBH37RV_THRESYN-PWY superpathway of L-threonine biosynthesis
BioCyc:ECOL316407_DAPLYSINESYN-PWY lysine biosynthesis I
BioCyc:ECOL316407_MET-SAM-PWY superpathway of S-adenosyl-L-methionine biosynthesis
BioCyc:CORYNE_METSYN-PWY L-homoserine and L-methionine biosynthesis
BioCyc:CORYNE_PWY-5345 superpathway of L-methionine biosynthesis (by sulfhydrylation)
BioCyc:CORYNE_THRESYN-PWY superpathway of L-threonine biosynthesis
BioCyc:META_PWY-5347 superpathway of L-methionine biosynthesis (transsulfuration)
BioCyc:ECO_MET-SAM-PWY superpathway of S-adenosyl-L-methionine biosynthesis
BioCyc:ECO_THRESYN-PWY superpathway of L-threonine biosynthesis
BioCyc:YEAST_HOMOSERSYN-PWY homoserine biosynthesis
BioCyc:MTBCDC1551_MET-SAM-PWY superpathway of S-adenosyl-L-methionine biosynthesis
BioCyc:ECOL316407_HOMOSERSYN-PWY homoserine biosynthesis
BioCyc:ECOL316407_THRESYN-PWY threonine biosynthesis
BioCyc:CORYNE_PWY-5347 superpathway of L-methionine biosynthesis (transsulfuration)
BioCyc:CORYNE_HOMOSERSYN-PWY L-homoserine biosynthesis
BioCyc:META_METSYN-PWY superpathway of L-homoserine and L-methionine biosynthesis
BioCyc:META_P4-PWY superpathway of L-lysine, L-threonine and L-methionine biosynthesis I
BioCyc:META_THRESYN-PWY superpathway of L-threonine biosynthesis
BioCyc:META_MET-SAM-PWY superpathway of S-adenosyl-L-methionine biosynthesis
BioCyc:META_PWY-3001 superpathway of L-isoleucine biosynthesis I
BioCyc:ECO_HOMOSERSYN-PWY L-homoserine biosynthesis
BioCyc:ECO_PWY0-781 aspartate superpathway
BioCyc:ECO_METSYN-PWY superpathway of L-homoserine and L-methionine biosynthesis
BioCyc:MTBCDC1551_METSYN-PWY L-homoserine and L-methionine biosynthesis
BioCyc:MTBCDC1551_HOMOSERSYN-PWY L-homoserine biosynthesis
BioCyc:HPY_HOMOSERSYN-PWY L-homoserine biosynthesis
BioCyc:BSUB_MET-SAM-PWY superpathway of S-adenosyl-L-methionine biosynthesis
BioCyc:BSUB_HOMOSERSYN-PWY L-homoserine biosynthesis
BioCyc:CORYNE_PWY-3001 superpathway of L-isoleucine biosynthesis I
BioCyc:CORYNE_P4-PWY superpathway of L-lysine, L-threonine and L-methionine biosynthesis I
PathBank:SMP0000834 Methionine Biosynthesis
PathBank:SMP0000837 Threonine Biosynthesis
PathBank:SMP0000992 Secondary Metabolites: Threonine Biosynthesis from Aspartate
PathBank:SMP0002309 Cysteine Metabolism
PathBank:SMP0002434 Lysine Metabolism
PathBank:SMP0002439 Methionine Metabolism
PathBank:SMP0002310 Methionine Metabolism and Salvage