EC: 1.1.1.271

GDP-L-fucose synthase (GDP-beta-L-fucose:NADP+ 4-oxidoreductase (3,5-epimerizing))

enzyme lambda metabolic reaction experiment
- [GDP-L-fucose synthase(Enzyme) wildtype]

NADH + GDP_4_dehydro_D_rhamnose + H+ -> GDP_L_fucose + NAD+		 (( (Vmax * A) ) / (Km_A + A)) buffer: 50 mM Tris-HCl
PH: 7
Temperature: 37
- [GDP-L-fucose synthase(Enzyme) wildtype]

GDP_4_dehydro_D_rhamnose + H+ + NADH -> GDP_L_fucose + NAD+		 (( (Vmax * B) ) / (Km_B + B)) buffer: 50 mM Tris-HCl
PH: 7
Temperature: 37
uniprot:P32055 [GDP-L-fucose synthase(Enzyme) wildtype]

H+ + NADPH + GDP_4_dehydro_D_rhamnose -> NADP+ + GDP_L_fucose		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM MOPS, 100 mM NaCl, 10 mM DTT, 5 mM EDTA
PH: 7
Temperature: 37
uniprot:P32055 [GDP-L-fucose synthase(Enzyme) wildtype]

NADPH + H+ + GDP_4_dehydro_D_rhamnose -> GDP_L_fucose + NADP+		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM MOPS, 100 mM NaCl, 10 mM DTT, 5 mM EDTA
PH: 7
Temperature: 37
uniprot:P32055 [GDP-L-fucose synthase(Enzyme) wildtype]

H+ + NADH + GDP_4_dehydro_D_rhamnose -> GDP_L_fucose + NAD+		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM MOPS, 100 mM NaCl, 10 mM DTT, 5 mM EDTA
PH: 7
Temperature: 37

Pathways

pathway id name
BioCyc:MOUSE_PWY-66 GDP-L-fucose biosynthesis I (from GDP-D-mannose)
BioCyc:ECOL316407_COLANSYN-PWY colanic acid building blocks biosynthesis
BioCyc:MOUSE_COLANSYN-PWY colanic acid building blocks biosynthesis
BioCyc:ECOL316407_PWY-66 GDP-L-fucose biosynthesis I (from GDP-D-mannose)
BioCyc:HUMAN_PWY-66 GDP-L-fucose biosynthesis I (from GDP-D-mannose)
BioCyc:ARA_PWY-66 GDP-L-fucose biosynthesis I (from GDP-D-mannose)
PathBank:SMP0000842 Mannose Metabolism
PathBank:SMP0000906 Amino Sugar and Nucleotide Sugar Metabolism II
PathBank:SMP0000967 Colanic Acid Building Blocks Biosynthesis
Plant Reactome:R-OSA-2883407 Carbohydrate metabolism
Plant Reactome:R-OSA-1119620 GDP-L-fucose biosynthesis I (from GDP-D-mannose)
Plant Reactome:R-OSA-2744345 Metabolism and regulation
Reactome:R-CEL-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-DDI-597592 Post-translational protein modification
Reactome:R-DDI-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-DME-597592 Post-translational protein modification
Reactome:R-DME-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-HSA-392499 Metabolism of proteins
Reactome:R-HSA-597592 Post-translational protein modification
Reactome:R-MMU-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-CEL-392499 Metabolism of proteins
Reactome:R-CEL-597592 Post-translational protein modification
Reactome:R-CEL-446203 Asparagine N-linked glycosylation
Reactome:R-CEL-6787639 GDP-fucose biosynthesis
Reactome:R-DDI-392499 Metabolism of proteins
Reactome:R-DDI-446203 Asparagine N-linked glycosylation
Reactome:R-DME-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-HSA-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-MMU-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-CEL-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-DDI-446219 Synthesis of substrates in N-glycan biosythesis
Reactome:R-DME-392499 Metabolism of proteins
Reactome:R-HSA-446203 Asparagine N-linked glycosylation
Reactome:R-HSA-6787639 GDP-fucose biosynthesis
Reactome:R-MMU-392499 Metabolism of proteins
Reactome:R-MMU-597592 Post-translational protein modification
Reactome:R-DDI-6787639 GDP-fucose biosynthesis
Reactome:R-DME-446203 Asparagine N-linked glycosylation
Reactome:R-DME-6787639 GDP-fucose biosynthesis
Reactome:R-HSA-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Reactome:R-MMU-446203 Asparagine N-linked glycosylation
Reactome:R-MMU-6787639 GDP-fucose biosynthesis
PathBank:SMP0000064 Fructose and Mannose Degradation
PathBank:SMP0000725 Fructose Intolerance, Hereditary
PathBank:SMP0012444 Mannose Metabolism
PathBank:SMP0000561 Fructosuria