EC: 1.17.1.8

4-hydroxy-tetrahydrodipicolinate reductase ((S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate:NAD(P)+ 4-oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:P04036 [Pyridine 2,6-dicarboxylate]

L_2,3_Dihydrodipicolinate + H+ + NADPH -> 2,3,4,5_Tetrahydrodipicolinate + NADP+		 (( (V * A) ) / (K + (A * (1 + (I / Kii))))) buffer: 100 mM Hepes, 100 mM L-Aspartic-beta-semialdehyde, 1 mM sodium pyruvate, 3.3 µg/ml dihydrodipicolinate synthase, 50% (v/v) glycerol
PH: 7.8
Temperature: 25
uniprot:P04036 [4-hydroxy-tetrahydrodipicolinate reductase(Enzyme) wildtype]

H+ + NADPH + L_2,3_Dihydrodipicolinate -> NADP+ + 2,3,4,5_Tetrahydrodipicolinate		 (( (( (V * A) ) * B) ) / (( (( (( (Ka * B) ) + (Kb * A)) ) + (Kia * Kb)) ) + (A * B))) buffer: 100 mM Hepes, 100 mM L-Aspartic-beta-semialdehyde, 1 mM sodium pyruvate, 3.3 µg/ml dihydrodipicolinate synthase, 50% (v/v) glycerol
PH: 7.8
Temperature: 25
uniprot:P04036 [Pyridine 2,6-dicarboxylate]

L_2,3_Dihydrodipicolinate + NADPH + H+ -> 2,3,4,5_Tetrahydrodipicolinate + NADP+		 (( (V * A) ) / (( (K * (1 + (I / Kis))) ) + A)) buffer: 100 mM Hepes, 100 mM L-Aspartic-beta-semialdehyde, 1 mM sodium pyruvate, 3.3 µg/ml dihydrodipicolinate synthase, 50% (v/v) glycerol
PH: 7.8
Temperature: 25
uniprot:P04036 [4-hydroxy-tetrahydrodipicolinate reductase(Enzyme) wildtype]

L_2,3_Dihydrodipicolinate + H+ + NADPH -> 2,3,4,5_Tetrahydrodipicolinate + NADP+		 (( (V * A) ) / (( (K * (1 + (I / Kis))) ) + (A * (1 + (I / Kii))))) buffer: 100 mM Hepes, 100 mM L-Aspartic-beta-semialdehyde, 1 mM sodium pyruvate, 3.3 µg/ml dihydrodipicolinate synthase, 50% (v/v) glycerol
PH: 7.8
Temperature: 25
uniprot:P04036 [4-hydroxy-tetrahydrodipicolinate reductase(Enzyme) wildtype]

H+ + NADPH + L_2,3_Dihydrodipicolinate -> 2,3,4,5_Tetrahydrodipicolinate + NADP+		 (( (V * A) ) / (( (K * (1 + (I / Kis))) ) + A)) buffer: 100 mM Hepes, 100 mM L-Aspartic-beta-semialdehyde, 1 mM sodium pyruvate, 3.3 µg/ml dihydrodipicolinate synthase, 50% (v/v) glycerol
PH: 7.8
Temperature: 25

Pathways

pathway id name
BioCyc:META_PWY0-781 aspartate superpathway
BioCyc:VCHO_P4-PWY superpathway of L-lysine, L-threonine and L-methionine biosynthesis I
BioCyc:HPY_DAPLYSINESYN-PWY L-lysine biosynthesis I
BioCyc:ECOL316407_P4-PWY superpathway of lysine, threonine and methionine biosynthesis I
BioCyc:ECOL316407_PWY0-781 aspartate superpathway
BioCyc:CORYNE_PWY0-781 aspartate superpathway
BioCyc:GCF_000013425_PWY-2941 L-lysine biosynthesis II
BioCyc:META_PWY-2942 L-lysine biosynthesis III
BioCyc:META_DAPLYSINESYN-PWY L-lysine biosynthesis I
BioCyc:ECO_P4-PWY superpathway of L-lysine, L-threonine and L-methionine biosynthesis I
BioCyc:ECO_DAPLYSINESYN-PWY L-lysine biosynthesis I
BioCyc:ARA_PWY-5097 L-lysine biosynthesis VI
BioCyc:CAULO_DAPLYSINESYN-PWY lysine biosynthesis I
BioCyc:ANTHRA_P4-PWY superpathway of L-lysine, L-threonine and L-methionine biosynthesis I
BioCyc:ECOL316407_DAPLYSINESYN-PWY lysine biosynthesis I
BioCyc:CORYNE_PWY-2942 L-lysine biosynthesis III
BioCyc:CORYNE_DAPLYSINESYN-PWY L-lysine biosynthesis I
BioCyc:META_PWY-2941 L-lysine biosynthesis II
BioCyc:ARA_PWY-724 superpathway of L-lysine, L-threonine and L-methionine biosynthesis II
BioCyc:VCHO_PWY0-781 aspartate superpathway
BioCyc:VCHO_DAPLYSINESYN-PWY L-lysine biosynthesis I
BioCyc:ANTHRA_PWY0-781 aspartate superpathway
BioCyc:BSUB_PWY-2941 L-lysine biosynthesis II
BioCyc:META_P4-PWY superpathway of L-lysine, L-threonine and L-methionine biosynthesis I
BioCyc:ECO_PWY0-781 aspartate superpathway
BioCyc:MTBH37RV_DAPLYSINESYN-PWY L-lysine biosynthesis I
BioCyc:ANTHRA_DAPLYSINESYN-PWY L-lysine biosynthesis I
BioCyc:SYNEL_PWY-5097 L-lysine biosynthesis VI
BioCyc:CORYNE_P4-PWY superpathway of L-lysine, L-threonine and L-methionine biosynthesis I
PathBank:SMP0000794 Lysine Biosynthesis
PathBank:SMP0063480 Monobactam Biosynthesis
PathBank:SMP0002434 Lysine Metabolism
PathBank:SMP0121266 Lysine Biosynthesis